Dynamics and Mechanism of CRISPR systems
Our lab uses computational methods to unravel the function and improve biological applications of CRISPR genome-editing machineries. So far, we described a mechanism for RNA binding and the association with the nucleic acids, and we delivered also molecular details on the off-target effects. We revealed an intriguing mechanism of allosteric regulation and the activation process of the HNH domain. We also established the atomic level details of the catalysis, a fundamental step for the editing of the genome. More research is yet to come!
 Caslino et al. Accepted ACS Catal. 2020.  Saha et al. Accepted JCIM 2020.  Nierzwicki et al. WIREs Comp Mol Sci Accepted 2020.  East K. et al. J. Am. Chem. Soc. 2020.  Palermo G. J. Chem. Inf. Model. 2019.  Ricci C. G. et al. ACS Cent. Sci. 2019.  Palermo G. Chem 2019.  Palermo G. et al. Q. Rev. Biophys. 2018.  Palermo G. et al. J. Am. Chem. Soc. 2017.  Palermo G. et al. Proc. Natl. Acad. Sci. USA, 2017.  Palermo G. et al. ACS Cent. Sci. 2016.
Read about the invisible dance of CRISPR-Cas9 in Physics Today!