Dynamics and Mechanism of CRISPR systems

Our lab uses computational methods to unravel the function and improve biological applications of CRISPR genome-editing machineries. So far, we described a mechanism for RNA binding and the association with the nucleic acids, and we delivered also molecular details on the off-target effects. We revealed an intriguing mechanism of allosteric regulation and the activation process of the HNH domain. We also established the atomic level details of the catalysis, a fundamental step for the editing of the genome. More research is yet to come! 


[1] Caslino et al. Accepted ACS Catal. 2020. [2] Saha et al. Accepted JCIM 2020. [3] Nierzwicki et al. WIREs Comp Mol Sci Accepted 2020. [4] East K. et al. J. Am. Chem. Soc. 2020. [5] Palermo G. J. Chem. Inf. Model. 2019. [6] Ricci C. G. et al. ACS Cent. Sci. 2019. [7] Palermo G. Chem 2019. [8] Palermo G. et al. Q. Rev. Biophys. 2018. [9] Palermo G. et al. J. Am. Chem. Soc. 2017. [10] Palermo G. et al. Proc. Natl. Acad. Sci. USA, 2017. [11] Palermo G. et al. ACS Cent. Sci. 2016.

                                                                Read about the invisible dance of CRISPR-Cas9 in Physics Today